FLOW CYTOFLUOROMETRY
\flˈə͡ʊ sˌa͡ɪtəflʊ͡əɹˈɒmətɹi], \flˈəʊ sˌaɪtəflʊəɹˈɒmətɹi], \f_l_ˈəʊ s_ˌaɪ_t_ə_f_l_ʊə_ɹ_ˈɒ_m_ə_t_ɹ_i]\
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Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
By DataStellar Co., Ltd
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dust storm
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